Studio dell’insediamento protostorico in un’area della Sardegna centro-occidentale tramite strumenti GIS ed analisi multivariate

The authors analyze, as a sample-area, the region that includes the municipality of Mogoro, in central-western Sardinia, with the objective to reconstruct, through the study of the settlements and their relationships, some economic and social aspects of the human groups of nuragic culture that inhabited this area between the 18th and the 8th century BC. The territory is located at the foot of Monte Arci, along the Mogoro river that runs through the southern part of the plain of the Campidano of Oristano. The area has been intensively investigated from the half of the past century; an in-depth stratigraphic investigation was carried out since 1994 near the nuragic site of Cuccurada, the main center of an articulated territorial system including a rich network of monuments related to the nuragic civilization. The results are illustrated through various research methods: GIS, with the application of spatial analysis tools, and multivariate analysis (cluster and principal components analysis) that allowed to set out new hypotheses on occupation and populating dynamics and to identify among pre-historical monuments one or more homogeneous and distinguishable groups, resulting from a database in which geomorphological characteristics are recorded. A hierarchical organization and a specific criterion for exploiting and monitoring the landscape have been developed, in which settlement choices depend on functionality criteria, having nuraghi and villages a key role on the strategic control of the territor

The gustin (CA6) gene polymorphism, rs2274333 (A/G), as a mechanistic link between PROP tasting and fungiform taste papilla density and maintenance

Taste sensitivity to PROP varies greatly among individuals and is associated with polymorphisms in the bitter receptor gene TAS2R38, and with differences in fungiform papilla density on the anterior tongue surface. Recently we showed that the PROP non-taster phenotype is strongly associated with the G variant of polymorphism rs2274333 (A/G) of the gene that controls the salivary trophic factor, gustin. The aims of this study were 1) to investigate the role of gustin gene polymorphism rs2274333 (A/G), in PROP sensitivity and fungiform papilla density and morphology, and 2) to investigate the effect of this gustin gene polymorphism on cell proliferation and metabolic activity. Sixty-four subjects were genotyped for both genes by PCR techniques, their PROP sensitivity was assessed by scaling and threshold methods, and their fungiform papilla density, diameter and morphology were determined. In vitro experiments examined cell proliferation and metabolic activity, following treatment with saliva of individuals with and without the gustin gene mutation, and with isolated protein, in the two iso-forms. Gustin and TAS2R38 genotypes were associated with PROP threshold (p=0.0001 and p=0.0042), but bitterness intensity was mostly determined by TAS2R38 genotypes (p<0.000001). Fungiform papillae densities were associated with both genotypes (p<0.014) (with a stronger effect for gustin; p=0.0006), but papilla morphology was a function of gustin alone (p<0.0012). Treatment of isolated cells with saliva from individuals with the AA form of gustin or direct application of the active iso-form of gustin protein increased cell proliferation and metabolic activity (p<0.0135). These novel findings suggest that the rs2274333 polymorphism of the gustin gene affects PROP sensitivity by acting on fungiform papilla development and maintenance, and could provide the first mechanistic explanation for why PROP super-tasters are more responsive to a broad range of oral stimul